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Part:BBa_J100028:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2011-08-30)

placeholder insert for BsaI Golden Gate Assembly of promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 858
    Illegal PstI site found at 872
    Illegal NotI site found at 7
    Illegal NotI site found at 865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 858
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 858
    Illegal PstI site found at 872
    Illegal AgeI site found at 730
    Illegal AgeI site found at 842
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 135
    Illegal BsaI.rc site found at 34


Design Notes

Had to avoid BsaI sites and BbsI sites as well as all biobrick restriction sites.

Below is a picture of what this part will look like when digested with Bsa I. TT represents the transcriptional terminator Part:BBa_B0014 J100028.png

Source

Synthesized de novo using DNA sequences from parts in the registry. Typical scars are absent from this DNA.

References